P-104: In Vivo Study of Follicogenesis and Estradiol Level in Mouse Exposed To Forced Swimming

Background: Strenuous exercise and temperature outside the optimum are associated with hypothalamic dysfunction and therefore disruption of fertility in females. The aim of this study was to evaluate serum Estradiol level and follicogenesis in adult mice exposed to forced swimming in different temperature waters. Materials and Methods: Adult female mice were randomly divided into four groups as: A. control, without exercise, B. swim group in 23°C water, C. swim group in 12°C water and D. swim group in 36°C water. All animals were swimming for 6 minutes daily for 5 days/week for 14 days, except controls. The mean values of the serum Estradiol level were measured by Elisa and follicogenesis was studied histologically. Results: Swimming in groups of B and D caused to reduction in serum estradiol levels in compare with controls (p<0.05). The numbers of Primary, Secondary and Tertiary follicules were significantly reduced in all swimming groups in compare with controls (p<0.05). Reduced follicogenesis and regression of ovary in group D (36°C) were more obvious. Conclusion: These data suggests that forced swimming as a physical stress disrupt HPG Axis and follicogenesis. Water temperature is also effective in ovarian function.